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VD-Ab-0009-100μg 425 USD

Anti-Akt1 pAb(Protein Kinase B)


Synonyms:
Anti-Protein Kinase B, PKB.

Background:
Akt1, initially identified as the cellular homolog to the retro-viral oncogene v-Akt, is part of the phosphatidyl 3’-kinase (PI3K)-Akt signaling pathway that is activated by diverse cellular stimuli and regulates critical cellular functions such as cell growth, proliferation, and survival. Following phosphorylation of the second messenger PIP2 by PI3K, Akt1 translocates to the cell membrane where it is activated by phosphoinositide-dependent kinase (PDK) 1 and PDK2. The active Akt1 is then able to phosphorylate and activate its substrates, including those that are important for cell proliferation and survival such as TOR and the Bcl-2 homolog Bad. Negative regulation of the PI3K-Akt signaling pathway is mainly accomplished by the lipid phosphatase activity of PTEN, which catalyzes the conversion of PIP3 to PIP2, thereby preventing the activation of Akt1. Inactivation of this gene often results in excessive Akt1 activity, often leading to the formation of malignant tumors.

Specificity:
Recognizes Akt1.

Positive Control:
Human liver cell lysate.

Species Reactivity:
Human, mouse and rat. Other species not tested.

Host:
Rabbit

Immunogen:
A 16 amino acid peptide from near the aminoterminus of human Akt1 (GenBank Accession No. AAH00479).

Format:
100 μg of polyclonal antibody in PBS containing 0.02% sodium azide. Purified by immunoaffinity chromatography.

Storage:
Store at 4°C.

Applications and Suggested Dilutions:
Western blot: Use at 1 μg/mL.
Immunohistochemistry: Use at ~2 μg/mL.
The optimal dilution for a specific application should be determined by the researcher.

Limitations:
For in vitro research use only. Not for use in diagnostics or in humans.

Warranty:
No warranties, expressed or implied, are made regarding the use of this product. VDM Biochemicals is not liable for any damage, personal injury, or economic loss caused by this product.