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Ac-IETD-AFC (CAS 211990-57-7)

Product specification

Formula: C31H38F3N5O12
MW: 729.65
Purity: ≥97% (HPLC)
Appearance: Lyophilized solid.
Solubility: Dimethylsulfoxide (DMSO) to 50 mg/ml.
Storage: −20°C; Shipped on dry ice
CAS Number: 211990-57-7
Synonyms: N-acetyl-Ile-Glu-Thr-Asp-AFC (7-amino-4-trifluoromethyl coumarin)
Cat.number: VD-NP-0054

Product Description

Fluorogenic substrate for caspases-6 (Mch2), -8 (FLICE), -10 (Mch4) and related cysteine proteases. Similar to Ac-IETD-AMC but the AFC fluorophore has a greater Stokes’ shift upon cleavage. Reaction can be monitored quantitatively or visually by a blue to green shift in fluorescence upon cleavage.

Certificate of Analysis (PDF):

Product References
1. N.A. Thornberry Methods Enzymol. 1994 244 615 
2. R. Datta et al. J. Biol. Chem. 1997 272 1965
3. D.W. Nicholson et al. Nature (Lond.) 1995 376 37
4. Y.A. Lazebnik et al. Nature (Lond.) 1994 371 346