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Ac-IETD-AFC (CAS 211990-57-7)




Product specification

Formula: C31H38F3N5O12
MW: 729.65
Purity: ≥97% (HPLC)
Appearance: Lyophilized solid.
Solubility: Dimethylsulfoxide (DMSO) to 50 mg/ml.
Storage: −20°C; Shipped on dry ice
CAS Number: 211990-57-7
Synonyms: N-acetyl-Ile-Glu-Thr-Asp-AFC (7-amino-4-trifluoromethyl coumarin)
Cat.number: VD-NP-0054

Product Description

Fluorogenic substrate for caspases-6 (Mch2), -8 (FLICE), -10 (Mch4) and related cysteine proteases. Similar to Ac-IETD-AMC but the AFC fluorophore has a greater Stokes’ shift upon cleavage. Reaction can be monitored quantitatively or visually by a blue to green shift in fluorescence upon cleavage.

 
Certificate of Analysis (PDF):



Product References
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2. R. Datta et al. J. Biol. Chem. 1997 272 1965
3. D.W. Nicholson et al. Nature (Lond.) 1995 376 37
4. Y.A. Lazebnik et al. Nature (Lond.) 1994 371 346