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VD-Ab-0004-100μg 460 USD

ADAM10(C-terminus)


Background:
Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease- disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Specificity:
This antibody recognizes ADAM10.

Species Reactivity:
Human, mouse, and rat. Other species not
tested.

Host:
Rabbit

Ig Isotype:
IgG

Immunogen:
A peptide corresponding to amino acids 732 to 748 of human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid.

Format:
200 μL at 500 μg/mL polyclonal antibody in PBS containing 0.02% sodium azide. Purified by ion exchange chromatography.

Storage:
Store at 4°C.

Applications and Suggested Dilutions:
Immunohistochemistry: Use at 2 μg/mL.
Western blot: Use at a 1:500-1:2,000 dilution.
The optimal dilution for a specific application should be determined by the researcher.

Positive Control:
Jurkat whole cell lysate. An approximately 85kDa band, which may represent precursor, can be detected.

Figure 1.
Immunohistochemical staining of K562 cells using anti-ADAM10 at 2 μg/mL.

Limitations:
For in vitro research use only. Not for use in diagnostics or in humans.

Warranty:
No warranties, expressed or implied, are made regarding the use of this product. VDM Biochemicals is not liable for any damage, personal injury, or economic loss caused by this product.