Z-GLF-CMK

Description

Z-Gly-Leu-Phe Chloromethyl Ketone (Z-GLF-CMK, Z=benzyloxycarbonyl) is a cell permeable inhibitor of Granzyme B.1 Granzyme B is a serine protease found in lytic granules of cytotoxic lymphocytes and plays a role in cell death or apoptosis. Granzymes A and B are the most abundant granzymes and are expressed earlier than other granzymes. Granzyme B is an agent for rapid DNA fragmentation and can cleave several procaspases to activate the corresponding caspase. The Asp primary substrate specificity of granzyme B, also found in caspases, is unusual among proteases. Lamin B is another substrate for granzyme B. CMK is a trapping group responsible for irreversible inhibition and is also non-cytotoxic. Inhibition occurs when the CMK group covalently bonds to the –OH of an serine adjacent residue, or the –SH of an adjacent cysteine residue, on the target protein.